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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Treinta y Tres. |
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Fecha : |
22/07/2018 |
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Actualizado : |
28/11/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
WEINROTH, M.D.; SCOTT, H.M.; NORBY, B.; LONERAGAN, G.H.; NOYES, N.R.; ROVIRA, P.J.; DOSTER, E.; YANG, X.; WOERNER, D.R.; MORLEY, P.S.; BELK, K.E. |
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Afiliación : |
M.D. WEINROTH, Universidad de Colorado State (CSU)/ Department of Animal Science (Meat Program); H.M. SCOTT, Universidad de Texas A&M; B. NORBY, Universidad de Michigan State (MSU); G.H. LONERAGAN, Texas Tech University; N.R. NOYES, Universidad de Colorado State (CSU); PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. DOSTER, Universidad de Colorado State (CSU); X. YANG, Universidad de California Davis (UCD); D.R. WOERNER, Universidad de Colorado State (CSU)/ Department of Animal Science (Meat Program); P. S. MORLEY, Universidad de Colorado State (CSU); K. E. BELK, Universidad de Colorado State (CSU)/ Department of Animal Science (Meat Program). |
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Título : |
Effects of ceftiofur and chlortetracycline on the resistomes of feedlot cattle. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Applied and Environmental Microbiology, July 2018, vol. 84, no.13, e00610-18. |
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Descripción física : |
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
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ISSN : |
0099-2240 |
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DOI : |
10.1128/AEM.00610-18 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 15 March 2018. / Accepted 24 April 2018. / Accepted manuscript posted online 4 May 2018. OPEN ACCESS. |
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Contenido : |
ABSTRACT.
Treatment of food-producing animals with antimicrobial drugs (AMD) is controversial because of concerns regarding promotion of antimicrobial resistance (AMR). To investigate this concern, resistance genes in metagenomic bovine fecal samples during a clinical trial were analyzed to assess the impacts of treatment on beef feedlot cattle resistomes. Four groups of cattle were exposed, using a 2-by-2 factorial design, to different regimens of antimicrobial treatment. Injections of ceftiofur crystalline- free acid (a third-generation cephalosporin) were used to treat all cattle in treatment pens or only a single animal, and either chlortetracycline was included in the feed of all cattle in a pen or the feed was untreated. On days 0 and 26, respectively, pre- and posttrial fecal samples were collected, and resistance genes were characterized using shotgun metagenomics. Treatment with ceftiofur was not associated with changes to ?-lactam resistance genes. However, cattle fed chlortetracycline had a significant increase in relative abundance of tetracycline resistance genes. There was also an increase of an AMR class not administered during the study, which is a possible indicator of coselection of resistance genes. Samples analyzed in this study had previously been evaluated by culture characterization (Escherichia coli and Salmonella) and quantitative PCR (qPCR) of metagenomic fecal DNA, which allowed comparison of results with this study. In the majority of samples, genes that were selectively enriched through culture and qPCR were not identified through shotgun metagenomic sequencing in this study, suggesting that changes previously documented did not reflect changes affecting the majority of bacterial genetic elements found in the predominant fecal resistome.
© 2018 American Society for Microbiology. MenosABSTRACT.
Treatment of food-producing animals with antimicrobial drugs (AMD) is controversial because of concerns regarding promotion of antimicrobial resistance (AMR). To investigate this concern, resistance genes in metagenomic bovine fecal samples during a clinical trial were analyzed to assess the impacts of treatment on beef feedlot cattle resistomes. Four groups of cattle were exposed, using a 2-by-2 factorial design, to different regimens of antimicrobial treatment. Injections of ceftiofur crystalline- free acid (a third-generation cephalosporin) were used to treat all cattle in treatment pens or only a single animal, and either chlortetracycline was included in the feed of all cattle in a pen or the feed was untreated. On days 0 and 26, respectively, pre- and posttrial fecal samples were collected, and resistance genes were characterized using shotgun metagenomics. Treatment with ceftiofur was not associated with changes to ?-lactam resistance genes. However, cattle fed chlortetracycline had a significant increase in relative abundance of tetracycline resistance genes. There was also an increase of an AMR class not administered during the study, which is a possible indicator of coselection of resistance genes. Samples analyzed in this study had previously been evaluated by culture characterization (Escherichia coli and Salmonella) and quantitative PCR (qPCR) of metagenomic fecal DNA, which allowed comparison of results with this study. In the majority of samples, gen... Presentar Todo |
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Palabras claves : |
ANTIBIOTIC RESISTANCE; ANTIMICROBIAL AGENTS; CATTLE; METAGENOMICS; POSTANTIBIOTIC EFFECT. |
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Thesagro : |
FEEDLOT. |
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Asunto categoría : |
--
L02 Alimentación animal |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12027/1/e00610-18.full.pdf
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Marc : |
LEADER 03064naa a2200361 a 4500
001 1058828
005 2018-11-28
008 2018 bl uuuu u00u1 u #d
022 $a0099-2240
024 7 $a10.1128/AEM.00610-18$2DOI
100 1 $aWEINROTH, M.D.
245 $aEffects of ceftiofur and chlortetracycline on the resistomes of feedlot cattle.$h[electronic resource]
260 $c2018
300 $cThis is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
500 $aArticle history: Received 15 March 2018. / Accepted 24 April 2018. / Accepted manuscript posted online 4 May 2018. OPEN ACCESS.
520 $aABSTRACT. Treatment of food-producing animals with antimicrobial drugs (AMD) is controversial because of concerns regarding promotion of antimicrobial resistance (AMR). To investigate this concern, resistance genes in metagenomic bovine fecal samples during a clinical trial were analyzed to assess the impacts of treatment on beef feedlot cattle resistomes. Four groups of cattle were exposed, using a 2-by-2 factorial design, to different regimens of antimicrobial treatment. Injections of ceftiofur crystalline- free acid (a third-generation cephalosporin) were used to treat all cattle in treatment pens or only a single animal, and either chlortetracycline was included in the feed of all cattle in a pen or the feed was untreated. On days 0 and 26, respectively, pre- and posttrial fecal samples were collected, and resistance genes were characterized using shotgun metagenomics. Treatment with ceftiofur was not associated with changes to ?-lactam resistance genes. However, cattle fed chlortetracycline had a significant increase in relative abundance of tetracycline resistance genes. There was also an increase of an AMR class not administered during the study, which is a possible indicator of coselection of resistance genes. Samples analyzed in this study had previously been evaluated by culture characterization (Escherichia coli and Salmonella) and quantitative PCR (qPCR) of metagenomic fecal DNA, which allowed comparison of results with this study. In the majority of samples, genes that were selectively enriched through culture and qPCR were not identified through shotgun metagenomic sequencing in this study, suggesting that changes previously documented did not reflect changes affecting the majority of bacterial genetic elements found in the predominant fecal resistome. © 2018 American Society for Microbiology.
650 $aFEEDLOT
653 $aANTIBIOTIC RESISTANCE
653 $aANTIMICROBIAL AGENTS
653 $aCATTLE
653 $aMETAGENOMICS
653 $aPOSTANTIBIOTIC EFFECT
700 1 $aSCOTT, H.M.
700 1 $aNORBY, B.
700 1 $aLONERAGAN, G.H.
700 1 $aNOYES, N.R.
700 1 $aROVIRA, P.J.
700 1 $aDOSTER, E.
700 1 $aYANG, X.
700 1 $aWOERNER, D.R.
700 1 $aMORLEY, P.S.
700 1 $aBELK, K.E.
773 $tApplied and Environmental Microbiology, July 2018, vol. 84, no.13, e00610-18.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
03/10/2018 |
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Actualizado : |
04/10/2018 |
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Tipo de producción científica : |
Serie FPTA |
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Autor : |
VIDAL, S.; GALLINO, J.P.; RIVERO, Y.; RUIBAL, C.; FLEITAS, L.; CASTILLO, A.; CERETTA, S.; CASARETTO, E.; BORSANI, O. |
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Afiliación : |
SABINA VIDAL, Universidad de la República (UdelaR)/ Facultad de Ciencias; JUAN PABLO GALLINO, Universidad de la República (UdelaR)/ Facultad de Ciencias; YAILY RIVERO, Universidad de la República (UdelaR)/ Facultad de Ciencias; CECILIA RUIBAL, Universidad de la República (UdelaR)/ Facultad de Ciencias; LUCIANA FLEITAS, Universidad de la República (UdelaR)/ Facultad de Ciencias; ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SERGIO EDUARDO CERETTA SORIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ESTEBAN CASARETTO, Universidad de la República (UdelaR)/ Facultad de Agronomía; OMAR BORSANI, Universidad de la República (UdelaR)/ Facultad de Agronomía. |
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Título : |
Biotecnología aplicada al mejoramiento genético de la soja. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Montevideo (UY): INIA, 2018. |
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Páginas : |
37 p. |
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Serie : |
(Serie FPTA-INIA; 68) |
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ISBN : |
9978-9974-38-409-5 |
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ISSN : |
1688-924X |
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Idioma : |
Español |
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Notas : |
Proyecto FPTA-309: Biotecnología aplicada al mejoramiento genético de la soja. Período de ejecución: Mayo 2014-Noviembre 2017. Institución Ejecutora: Universidad de la República, Facultad de Ciencias. Responsable del Proyecto: Sabina Vidal. |
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Contenido : |
La soja (Glycine max L.) es uno de los cuatro cultivos de mayor importancia a nivel mundial. El objetivo general del proyecto fue desarrollar las capacidades a nivel nacional para incluir la
ingeniería genética en el repertorio de técnicas útiles al mejoramiento genético del cultivo de soja. En particular, el proyecto tuvo como objetivos desarrollar las capacidades en el país para
transformar soja y generar intragénicos de esta especie que incorporen genes involucrados en la tolerancia a sequía. |
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Palabras claves : |
CISGÉNESIS; MUTAGÉNESIS QUÍMICA; TRANSGÉNESIS. |
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Thesagro : |
BIOTECNOLOGÍA; FITOMEJORAMIENTO; INGENIERIA GENETICA; SOJA. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/11354/1/fpta-68-proy.309-2018.pdf
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Marc : |
LEADER 01611nam a2200337 a 4500
001 1059139
005 2018-10-04
008 2018 bl uuuu u00u1 u #d
022 $a1688-924X
100 1 $aVIDAL, S.
245 $aBiotecnología aplicada al mejoramiento genético de la soja.$h[electronic resource]
260 $aMontevideo (UY): INIA$c2018
300 $a37 p.
490 $a(Serie FPTA-INIA; 68)
500 $aProyecto FPTA-309: Biotecnología aplicada al mejoramiento genético de la soja. Período de ejecución: Mayo 2014-Noviembre 2017. Institución Ejecutora: Universidad de la República, Facultad de Ciencias. Responsable del Proyecto: Sabina Vidal.
520 $aLa soja (Glycine max L.) es uno de los cuatro cultivos de mayor importancia a nivel mundial. El objetivo general del proyecto fue desarrollar las capacidades a nivel nacional para incluir la ingeniería genética en el repertorio de técnicas útiles al mejoramiento genético del cultivo de soja. En particular, el proyecto tuvo como objetivos desarrollar las capacidades en el país para transformar soja y generar intragénicos de esta especie que incorporen genes involucrados en la tolerancia a sequía.
650 $aBIOTECNOLOGÍA
650 $aFITOMEJORAMIENTO
650 $aINGENIERIA GENETICA
650 $aSOJA
653 $aCISGÉNESIS
653 $aMUTAGÉNESIS QUÍMICA
653 $aTRANSGÉNESIS
700 1 $aGALLINO, J.P.
700 1 $aRIVERO, Y.
700 1 $aRUIBAL, C.
700 1 $aFLEITAS, L.
700 1 $aCASTILLO, A.
700 1 $aCERETTA, S.
700 1 $aCASARETTO, E.
700 1 $aBORSANI, O.
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